Multiple drug resistance patterns and plasmid profiles of non-typhi salmonellae in Turkey

Yildirmak, T
Yazgan, A
Özcengiz, Gülay
A total of 259 clinical isolates of nonrepetitive non-typhi salmonellae (NTS) were examined for antibiotic resistance patterns and plasmid content. The antibiotics used were amoxicillin-clavulanic acid (AMC), ampicillin (AM), aztreonam (ATM), carbenicillin (CB), cefixime (CFM), cefotaxime (CTX), cefoxitin (FOX), ceftazidime (CAZ), ceftriaxone (CRO), chloramphenicol (C), ciprofloxacin (CIP), gentamicin (GM), imipenem (IPM), ofloxacin (OFX), tetracycline (TE), trimethoprim-sulfomethoxazole (SXT). Multi-drug resistant (MDR) strains comprised 19.3 % of the total isolates (50/259) and almost all were S. typhimurium (49/50). Fifteen different patterns of resistance was observed, AM/CB/C/AMC/TE and AM/CB/C/AMC/SXT/GM/CTX/CRO/CAZ/CFM/ATM being the most frequent patterns. Twenty-eight out of 50 multiresistant isolates were found to contain at least one plasmid (mean five) and the size of the plasmids ranged between 1.7 and 158 kb. Plasmid profiles of multiresistant NTS strains were heterogenous as 21 different profiles were detected in a total of 28 plasmid-bearing isolates. No direct correlation was established between antibiotic resistance patterns and plasmid profiles.


Colicinogeny, lipopolysaccharide and outer membrane protein profiles of multi-drug resistant Salmonella typhimurium isolates from Turkey
İçgen, Bülent; Özcengiz, Gülay; Gültekin, Güzin Candan ( TÜBİTAK, 2002-01-01)
A total of 259 clinical isolates of non-repetitive non-typhi salmonellae (NTS) were previously examined for their antibiotic resistance patterns and plasmid contents. Multi-drug resistant strains comprised 19.3% (50/259) of the isolates and almost all were Salmonella typhimurium. In the present study, 35 of these multi-drug resistant S. typhimurium isolates were further characterized for colicinogeny, lipopolysaccharide (LPS) and outer membrane protein (OMP) profiles. Fourteen of the 35 (40%) isolates were ...
Development of recombinant vaccine candidates composed of ltka from mannheimia haemolytica A1 and PlpEC AND OmpH from Pasteurella Multocida A:3 against bovine respiratory disease
Çırçır, Ayça; Özcengiz, Gülay; Department of Biology (2014)
Mannheimia haemolytica A1 and Pasteurella multocida serotype A:3 are gramnegative bacterial pathogens which are considered the causative agents of bovine respiratory disease (BRD) or shipping fever in cattle. The foremost of several virulence factors of M. haemolytica A1 which allows lung colonization and establishment of infection is leukotoxin (Lkt). The others include adhesin, capsule, outer membrane proteins, and various proteases used for attachment, spreading and evading innate and adaptive host immun...
A new ZTL-type F-box functions as a positive regulator in disease resistance: VIGS analysis in barley against powdery mildew
Dagdas, Yasin Fatih; Dagdas, Gulay; Unver, Turgay; Akkaya, Mahinur (2009-01-01)
In our previous studies, we found a new Zeitlupe (ZTL) type F-box protein which is expressed at a higher level upon avirulent pathogen infection (Bozkurt et al., 2007). F-box proteins mark the proteins to be degraded through 26S proteasome system by ubiquitination. Since the information on the role of ubiquitin mediated proteolysis in disease responses is advancing rapidly, we sought to understand the way which F-box functions in resistance response as part of ubiquitin-proteasome pathway. Interestingly, in...
Identification of R Plasmids of Pseudomonas aeruginosa isolates from clinical sources by recombinant DNA techniques
İzgü, Kadri Fatih (1994-01-01)
The R-plasmids, which transfer resistance to p. aeruginosa strains agaiiıst generally used antibiotics in the treatmet of the infections caıısed by this gram (-) bacteria were investigated. The P. aeruginosa samples used in this study were isolat-ed from the clinical cases of Gülhane Military Medical Academy (GATA), such as throat, ear and urinary tract infections. Those clinical specimens of P. aeruginosa were tested far their sensitivities to fourteen different antibiotics that are used against P. aeurigo...
Analysis of a bac operon-silenced strain suggests pleiotropic effects of bacilysin in Bacillus subtilis
Ertekin, Ozan; Taskin, Ash Aras; Demir, Mustafa; Karataş, Ayten; Özcengiz, Gülay (Springer Science and Business Media LLC, 2020-04-01)
Bacilysin, as the simplest peptide antibiotic made up of only L-alanine and L-anticapsin, is produced and excreted by Bacillus subtilis under the control of quorum sensing. We analyzed bacilysin-nonproducing strain OGU1 which was obtained by bacA-targeted pMutin T3 insertion into the parental strain genome resulting in a genomic organization (bacA '::lacZ::erm::bacABCDEF) to form an IPTG-inducible bac operon. Although IPTG induction provided 3- to 5-fold increment in the transcription of bac operon genes, n...
Citation Formats
T. Yildirmak, A. Yazgan, and G. Özcengiz, “Multiple drug resistance patterns and plasmid profiles of non-typhi salmonellae in Turkey,” EPIDEMIOLOGY AND INFECTION, pp. 303–307, 1998, Accessed: 00, 2020. [Online]. Available: