Fe3+/SeO42- dual doped nano hydroxyapatite: A novel material for biomedical applications

Date

2018-01-01

Author

ALSHEMARY, AMMAR ZEIDAN GHAILAN
Pazarçeviren, Ahmet Engin
Tezcaner, Ayşen
Evis, Zafer

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

146
views

0
downloads

Dual ions substituted hydroxyapatite (HA) received attention from scientists and researchers in the biomedical field owing to their excellent biological properties. This paper presents a novel biomaterial, which holds potential for bone tissue applications. Herein, we have successfully incorporated ferric (Fe3+)/selenate ( SeO42-) ions into the HA structure (Ca10-x-yFey(PO4)(6-x)(SeO4)(x)(OH)(2-x-y)O-y) (Fe-SeHA) through a microwave refluxing process. The Fe-SeHA materials were characterized by X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and field emission scanning electron microscopy (FESEM). XRD and FTIR analyses revealed that Fe-SeHA samples were phase pure at 900 degrees C. FESEM images showed that formation of rod-like shaped particles was inhibited dramatically with increasing Fe3+ amount. The Vickers hardness (HV) test showed that hardness values increased with increasing Fe3+ concentrations. Optical spectra of Fe-SeHA materials contained broadband over (200-600) nm. In vitro degradation and bioactivity tests were conducted in simulated body fluid (SBF). The incorporation of Fe3+/ SeO42- ions into the HA structure resulted in a remarkably higher degradation rate along with intense growth of apatite granules on the surface of the Fe-SeHA discs with Ca/P ratio of 1.35-1.47. In vitro protein adsorption assay was conducted in fetal bovine serum (FBS) and it was observed that the adsorption of serum proteins on Fe-SeHA samples significantly increased with increasing Fe3+ concentration. In vitro cytotoxicity tests were performed with human fetal osteoblast (hFOB) cell line and the results demonstrated that hFOB cells attached and proliferated faster on the Fe-SeHA materials compared to pure HA showing that Fe-SeHA materials were cytocompatible. ALP activity and intracellular calcium of hFOB cells on 1Fe-SeHA discs were statistically higher than pure HA, suggesting that presence of Fe3+ ion supported osteogenic differentiation of hFOB cells. Our results suggest that 1Fe-SeHA (0.2M Fe3+/0.5MSeO42- co-doped HA) material could be considered as a promising candidate material for orthopedic applications. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 340-352, 2018.

Subject Keywords

Dual doped HA, Fe3+, SeO(4)(2-)ions, hFOB cells, hFOB cells, In vitro degradation, Novel biomaterial

URI

https://hdl.handle.net/11511/41711

Journal

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS

DOI

https://doi.org/10.1002/jbm.b.33838

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Collagen/PEO/gold nanofibrous matrices for skin tissue engineering Akturk, Omer; Keskin, Dilek (The Scientific and Technological Research Council of Turkey, 2016-01-01) As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing 14.27 ppm AuNPs (CM-Au) had the best nanofibrous morphology. CM-Au was cross-linked with glutaraldehyde vapor (CM-AuX). All groups were disrupted in collagenase in 2 h, but cross-linked groups and Matriderm (R) resisted hydrolytic degradation for 7 and 14 days, respectively. Due to its small pores a...
Collagen scaffolds with in situ-grown calcium phosphate for osteogenic differentiation of Wharton's jelly and menstrual blood stem cells Karadas, Ozge; Yucel, Deniz; Kenar, Halime; Kose, Gamze Torun; Hasırcı, Vasıf Nejat (2014-07-01) The aim of this research was to investigate the osteogenic differentiation potential of non-invasively obtained human stem cells on collagen nanocomposite scaffolds with in situ-grown calcium phosphate crystals. The foams had 70% porosity and pore sizes varying in the range 50-200 mu m. The elastic modulus and compressive strength of the calcium phosphate containing collagen scaffolds were determined to be 234.5 kPa and 127.1 kPa, respectively, prior to in vitro studies. Mesenchymal stem cells (MSCs) obtain...
Poly(ester-urethane) scaffolds: effect of structure on properties and osteogenic activity of stem cells Kızıltay, Aysel; San Roman, Julio; Sousa, Rui A.; Reis, Rui L.; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2015-08-01) The present study aimed to investigate the effect of structure (design and porosity) on the matrix stiffness and osteogenic activity of stem cells cultured on poly(ester-urethane) (PEU) scaffolds. Different three-dimensional (3D) forms of scaffold were prepared from lysine-based PEU using traditional salt-leaching and advanced bioplotting techniques. The resulting scaffolds were characterized by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), me...
Dielectric Analysis of Changes in Electric Properties of Doxorubicin Resistant K562 Leukemic Cells Through Electrorotation with 3 D Electrodes Garsha, Bahrieh; Erdem, Murat; Özgür, Ebru; Gündüz, Ufuk; Külah, Haluk (2013-10-31) In this study, dielectric characterization of multidrug resistant (MDR) K562 human leukemia cells was carried out using a MEMS-based electrorotation (ER) device with 3D electrodes. Different cell populations were utilized, which were resistant to 0.1, 0.3, and 0.5 μM doxorubicin. The ER devices with 3D quadruple electrodes (30 urn in height) were used, in order to eliminate the fringing field effect on the rotation of cells. Signals in phase quadrature were applied to the polynomial electrodes, to induce th...
Plasmacytoid Dendritic Cell Response to CpG ODN Correlates with CXCL16 Expression and Is Inhibited by ox-LDL Gürsel, Mayda; GÜRSEL, İHSAN (Hindawi Limited, 2013-01-01) Structurally distinct classes of synthetic CpG oligonucleotides (ODN) differentially activate human immune cells. K-type ODN trigger plasmacytoid dendritic cells (pDCs) to differentiate and produce TNF alpha. In contrast, D-type ODN stimulate large amounts of IFN alpha secretion from pDCs. The cell-surface receptor CXCL16 was previously shown to influence the nature and specificity of CpG ODN-induced immune activation. Here, we evaluated the expression and function of CXCL16 on pDC from healthy volunteers. ...

Citation Formats

A. Z. G. ALSHEMARY, A. E. Pazarçeviren, A. Tezcaner, and Z. Evis, “Fe3+/SeO42- dual doped nano hydroxyapatite: A novel material for biomedical applications,” JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, pp. 340–352, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41711.