Cellulose acetate based 3-dimensional electrospun scaffolds for skin tissue engineering applications

Skin defects that are not able to regenerate by themselves are among the major problems faced. Tissue engineering approach holds promise for treating such defects. Development of tissue-mimicking-scaffolds that can promote healing process receives an increasing interest in recent years. In this study, 3-dimensional electrospun cellulose acetate (CA) pullulan (PULL) scaffolds were developed for the first time. PULL was intentionally used to obtain 3D structures with adjustable height. It was removed from the electrospun mesh to increase the porosity and biostability. Different ratios of the polymers were electrospun and analyzed with respect to degradation, porosity, and mechanical properties. It has been observed that fiber diameter, thickness and porosity of scaffolds increased with increased PULL content, on the other hand this resulted with higher degradation of scaffolds. Mechanical strength of scaffolds was improved after PULL removal suggesting their suitability as cell carriers. Cell culture studies were performed with the selected scaffold group (CA/PULL: 50/50) using mouse fibroblastic cell line (L929). In vitro cell culture tests showed that cells adhered, proliferated and populated CA/PULL (50/50) scaffolds showing that they are cytocompatible. Results suggest that uncrosslinked CA/PULL (50/50) electrospun scaffolds hold potential for skin tissue engineering applications.


Natural origin bilayer pullulan-PHBV scaffold for wound healing applications
Dalgic, Ali Deniz; Koman, Ezgi; Karataş, Ayten; Tezcaner, Ayşen; Keskin, Dilek (2022-03-01)
Skin tissue loss that occurs by injury and diseases can turn into chronic wounds as a result of complications alongside infection. Chronic wounds fail to heal by themselves and need advanced treatments like engineered wound dressings and regenerative scaffolds. In this study, a novel, natural origin, bilayer electrospun scaffold was produced from pullulan (PUL) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) biopolymers. PHBV production by Cupriavidus necator bacterial strain was optimized and produced ...
Biocompatibility of Dead Sea Water and retinyl palmitate carrying poly(3-hydroxybutyrate-co-3-hydroxyvalerate) micro/nanoparticles designed for transdermal skin therapy
Eke, Gozde; Goni-de-Cerio, Felipe; Suarez-Merino, Blanca; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat (2015-09-01)
In this study, novel drug carriers were developed for the treatment of skin conditions such as psoriasis, aging, or ultraviolet damage using micro/nanocapsules and micro/nanospheres of poly(3-hydroxybutyrate-co-3-hydroxyvalerate). The sizes of the particles were in the micron range and were loaded with retinyl palmitate and Dead Sea Water. In some tests, MgCl2 was used as a substitute for Dead Sea Water for accurate determination of released ions of Dead Sea Water. Encapsulation efficiency and loading of wa...
A bilayer scaffold prepared from collagen and carboxymethyl cellulose for skin tissue engineering applications
Bektas, Cemile Kilic; Kimiz, Ilgin; ŞENDEMİR, AYLİN; Hasırcı, Vasıf Nejat; Hasırcı, Nesrin (2018-01-01)
Treatment of chronic skin wound such as diabetic ulcers, burns, pressure wounds are challenging problems in the medical area. The aim of this study was to design a bilayer skin equivalent mimicking the natural one to be used as a tissue engineered skin graft for use in the treatments of problematic wounds, and also as a model to be used in research related to skin, such as determination of the efficacy of transdermal bioactive agents on skin cells and treatment of acute skin damages that require immediate r...
Topical delivery of heparin with polymeric nanoparticles on electrospun nanofibers
Akolpoğlu, Duygu Deniz; Keskin, Dilek; Department of Biotechnology (2019)
Skin surrounds underlying organs and preserve the body against harmful microbial, thermal and mechanical effects and skin damages may give rise to crucial disorders or even death. Therefore, skin regeneration is one of the most important issues in tissue engineering. Search on more effective biomaterials that will enhance regeneration while enabling requirements of a healing skin site is an important issue. In this study, heparin was encapsulated in poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) a...
Addition of Selenium Nanoparticles to Electrospun Silk Scaffold Improves the Mammalian Cell Activity While Reducing Bacterial Growth
Chung, Stanley; Ercan, Batur; Roy, Amit K.; Webster, Thomas J. (2016-07-14)
Silk possesses many beneficial wound healing properties, and electrospun scaffolds are especially applicable for skin applications, due to their smaller interstices and higher surface areas. However, purified silk promotes microbial growth. Selenium nanoparticles have shown excellent antibacterial properties and are a novel antimicrobial chemistry. Here, electrospun silk scaffolds were doped with selenium nanoparticles to impart antibacterial properties to the silk scaffolds. Results showed significantly im...
Citation Formats
D. Atila, D. Keskin, and A. Tezcaner, “Cellulose acetate based 3-dimensional electrospun scaffolds for skin tissue engineering applications,” CARBOHYDRATE POLYMERS, pp. 251–261, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/48708.