A Puccinia striiformis f. sp. tritici secreted protein activates plant immunity at the cell surface

Download

10.1038-s41598-017-01100-z.pdf

Date

2017-4-25

Author

Dagvadorj, Bayantes
Duggan, Cian
Özketen, Ahmet Çaglar
Andaç, Ayşe
Bozkurt, Tolga Osman
Akkaya, Mahinur S.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

112
views

115
downloads

Pathogens secrete effector proteins to suppress host immunity, mediate nutrient uptake and subsequently enable parasitism. However, on non-adapted hosts, effectors can be detected as non-self by host immune receptors and activate non-host immunity. Nevertheless, the molecular mechanisms of effector triggered non-host resistance remain unknown. Here, we report that a small cysteine-rich protein PstSCR1 from the wheat rust pathogen Puccinia striiformis f. sp. tritici (Pst) activates immunity in the non-host solanaceous model plant Nicotiana benthamiana. PstSCR1 homologs were found to be conserved in Pst, and in its closest relatives, Puccinia graminis f. sp. tritici and Puccinia triticina. When PstSCR1 was expressed in N. benthamiana with its signal peptide, it provoked the plant immune system, whereas no stimulation was observed when it was expressed without its signal peptide. PstSCR1 expression in N. benthamiana significantly reduced infection capacity of the oomycete pathogens. Moreover, apoplast-targeted PstSCR1 triggered plant cell death in a dose dependent manner. However, in Brassinosteroid insensitive 1-Associated Kinase 1 (SERK3/BAK1) silenced N. benthamiana, cell death was remarkably decreased. Finally, purified PstSCR1 protein activated defence related gene expression in N. benthamiana. Our results show that a Pst-secreted protein, PstSCR1 can activate surface mediated immunity in non-adapted hosts and contribute to non-host resistance.

Subject Keywords

Pattern-recognition receptors, Membrane rafts, Effectors, Tomato, Expression, Complexes, Sequence, Biology, Vectors, Targets

URI

https://hdl.handle.net/11511/51585

Journal

Scientific Reports

DOI

https://doi.org/10.1038/s41598-017-01100-z

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

An effector ofPuccinia striiformisf. sp.triticitargets chloroplasts with a novel and robust targeting signal Andac, Ayse; Ozketen, Ahmet Caglar; Dagvadorj, Bayantes; Akkaya, Mahinur (Springer Science and Business Media LLC, 2020-08-01) Fungal pathogens secrete effector molecules into host plant cells to suppress host immunity to colonize plants. Ongoing efforts are being made to identify and characterize effector proteins in many fungal plant pathogens. Nevertheless, the precise biological and biochemical functions of many effectors, such as their trafficking from the pathogen to the host, have yet to be fully understood. In this study, we show that an effector candidate, matured PstCTE1 ofPuccinia striiformisf. sp.tritici, localizes to c...
C-4 ' Truncated carbocyclic formycin derivatives Zhou, Jian; Yang, Minmin; Akdağ, Akın; Schneller, Stewart W. (Elsevier BV, 2006-07-01) Formycin is a naturally occurring C-glycoside (C-nucleoside) that possesses antitumor, antibacterial, antifungal, and antiviral activity. In connection with our ongoing interest in the design and syntheses of C-nucleoside derived antiviral agents this report describes the preparation of carbocyclic formycin and its 7-hydroxy (oxo) analog lacking the C-4' hydroxylmethylene moiety in racemic form (4 and 6, respectively). An antiviral analysis of (+/-)-4 did not disclose any activity.
Immunotherapeutic applications of CpG ODN Gürsel, Mayda (2006-06-01) Bacterial DNA and synthetic oligodeoxynucleotides (ODN) expressing unmethylated CpG motifs stimulate the mammalian immune system to mount a rapid innate immune response. This response is characterized by the production of polyreactive IgM, immunomodulatory cytokines and chemokines. CpG ODN directly stimulate lymphocytes, natural killer cells and professional antigen-presenting cells (such as macrophages and dendritic cells). Owing to the strength and nature of this stimulation, CpG ODN are being harnessed f...
Partially Observable Gene Regulatory Network Control Without a Boundary on Horizon Erdogdu, Utku; Polat, Faruk; Alhajj, Reda (2012-11-09) Gene regulatory networks (GRNs) govern the protein transcription process in the cell and interactions among genes play a vital role in determining the biosynthesis rate of proteins. By using intervention techniques discovered by biological research it is possible to control a GRN, thus promoting or demoting the expression rate of a certain gene. In this work, this control task is studied in a partially observable setting where interventions lack perfect knowledge of the expression level of all genes. Moreov...
ARID3B expression in primary breast cancers and breast cancer-derived cell lines ERDOGAN, Ayse Selcen Oguz; OZDEMIRLER, Nihan; ÖYKEN, MERVE; ALPER, Murat; Erson Bensan, Ayşe Elif (2014-08-01) ARID3B (AT-rich interaction domain 3) is a member of the family of ARID proteins, which constitutes evolutionarily conserved transcription factors implicated in normal development, differentiation, cell cycle regulation and chromatin remodeling. In addition, ARID3B has been linked to cellular immortalization, epithelial-mesenchymal transition (EMT) and tumorigenesis. Given the emerging role of ARID3B in tumor development, we examined its expression in primary patient-derived breast cancer samples and breast...

Citation Formats

B. Dagvadorj, C. Duggan, A. Ç. Özketen, A. Andaç, T. O. Bozkurt, and M. S. Akkaya, “A Puccinia striiformis f. sp. tritici secreted protein activates plant immunity at the cell surface,” Scientific Reports, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/51585.