Mitochondrial DNA variation in honey bee (Apis mellifera L.) populations from Turkey

Kandemir, Irfan
Kence, Meral
Sheppard, Walter S.
Kence, Aykut
We have studied mitochondrial (mt) DNA variation in 334 honey bee colonies from 7 different geographic regions of Turkey. We have evaluated Dra I restriction profiles of the CO-I CO-II intergenic region, Hinf-I and Taq-1 restriction profiles of the CO-I gene and EcoR-1 restriction profiles of the whole mtDNA. We obtained three different mtDNA patterns by EcoR-1 digestion. The pattern typical for A. m. carnica/A. m. ligustica predominated throughout Turkey (327 colonies, 97.9%). We observed the pattern common in African subspecies only in Hatay province (6 colonies, 1.8%) and a previously unreported pattern in one colony from Balikesir province (0.3%). Dra-1 restriction analysis of the CO-I CO-II intergenic region yielded seven haplotypes. Haplotype 1 (TrDra-1) was the most common one found in Turkey, whereas haplotype 2 (TrDra-2) was widely distributed in Eastern Anatolia. Based on mitochondrial ND2 sequences taken from two samples collected in each region, bees from Hatay clustered with A. m. lamarckii and A. m. meda (morphological A and O lineages), while bees from central Anatolia clustered within the C morphological lineage group.


Genetic and morphometric variation in honeybee (Apis mellifera L.) populations of Turkey
Kandemir, I; Kence, M; Kence, Aykut (Springer Science and Business Media LLC, 2000-05-01)
Six enzyme systems were studied to determine the genetic variability in honeybee populations in Turkey. Ten morphometric characters were also measured to determine the extent of morphometric variation. Out of six enzyme systems, four were found to be polymorphic with 16 allozymes. The average heterozygosity was calculated as 0.072 +/- 0.007. Morphometric and electrophoretic variables were equally effective in discriminating honeybee populations. European and Anatolian honeybees were separated on the first a...
A comparative genetic analysis of the subterranean termite genus Reticulitermes (Isoptera : Rhinotermitidae)
Austin, JW; Szalanski, AL; Uva, P; Bagneres, AG; Kence, Aykut (Oxford University Press (OUP), 2002-11-01)
DNA sequencing analysis of the mitochondrial DNA cytochrome oxidase H (COII) region was used to examine genetic variation in the termite genus Reticulitermes Holmgren. We examined 21 species and subspecies from three continents. Sequencing of a 677-bp region of a 780-bp amplicon from 41 individuals and from 17 sequences obtained from GenBank revealed 221 polymorphic sites within the genus. Tajima-Nei distances from species ranged from 0.9 to 12.7%, and parsimony and maximum likelihood analysis revealed seve...
Test of independence in the Farlie-Gumbel-Morgenstern distribution
Guven, B (2003-01-01)
We consider the hypotheses; H-0 : theta = 0 vs. H-1 : theta greater than or equal to eta where theta is the dependence parameter of the Farlie-Gumbel-Morgenstren distribution and eta is an element of (0, 1]. A test, which maximizes the minimum power over the alternative hypothesis, is given for these hypotheses. The power function of this test is monotone increasing over the alternative hypothesis. Furthermore, the asymptotic distribution and the approximate power of the test are presented.
Molecular detection of Nosema ceranae and N. apis from Turkish honey bees
Whitaker, Justin; Szalanski, Allen L.; Kence, Meral (Springer Science and Business Media LLC, 2011-03-01)
Polymerase chain reaction specific for the rDNA marker for Nosema ceranae and Nosema apis was conducted on 84 Apis mellifera samples collected from 20 provinces in Turkey. N. ceranae was detected from three samples from the provinces of Artvin, Hatay, and Mugla. N. apis was detected in samples from the provinces of Sivas, Izmir, Bitlis and Gaziantep. All of the positive samples were from honey bees belonging to the 'C' lineage of A. mellifera. DNA sequencing analysis of the N. ceranae samples revealed that ...
Mitochondrial DNA Diversity of Modern, Ancient and Wild Sheep (Ovis gmelinii anatolica) from Turkey: New Insights on the Evolutionary History of Sheep
Demirci, Sevgin; Bastanlar, Evren Koban; Dagtas, Nihan Dilsad; Pişkin, Evangelia; ENGİN, ATİLLA; Ozer, Fusun; Yuncu, Eren; Dogan, Sukru Anil; TOGAN, İNCİ ZEHRA (2013-12-11)
In the present study, to contribute to the understanding of the evolutionary history of sheep, the mitochondrial (mt) DNA polymorphisms occurring in modern Turkish native domestic (n = 628), modern wild (Ovis gmelinii anatolica) (n = 30) and ancient domestic sheep from Oylum Hoyuk in Kilis (n = 33) were examined comparatively with the accumulated data in the literature. The lengths (75 bp/76 bp) of the second and subsequent repeat units of the mtDNA control region (CR) sequences differentiated the five hapl...
Citation Formats
I. Kandemir, M. Kence, W. S. Sheppard, and A. Kence, “Mitochondrial DNA variation in honey bee (Apis mellifera L.) populations from Turkey,” JOURNAL OF APICULTURAL RESEARCH, pp. 33–38, 2006, Accessed: 00, 2020. [Online]. Available: