EVALUATION OF IN VIVO EFFECTS OF VISCUM ALBUM L. AND ITS CONSTITUENT O-COUMARIC ACID ON BILE ACID SYNTHESIZING CYPS

Date

2019-01-01

Author

Ege, Tuba
Ozkan, Ayse Mine Gencler
Sena, Alaattin
Adalı, Orhan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

232
views

0
downloads

This research was designed to determine about the possible potency of medicinal plants Viscum album L. and its major polyphenolic ingredient, o-coumaric acid on rat liver cholesterol and bile acid metabolizing enzymes. Bioactive compounds of V. album extracts were identified using LC-MS technique. The water extracts of V. album (10 mg/kg) and o-coumaric acid (30 mg/kg) were injected intraperitoneally for 9 days. Then, in vivo effects of this plant and its major phenolic ingredient (o-coumaric acid) on rat liver cholesterol and bile acid metabolizing cytochromes P450s were analyzed by determining protein and mRNA expression levels using western blotting and qRT-PCR techniques, respectively. The results showed that V. album caused a 0.9 fold decrease in protein expression of CYP7A1 and CYP7B1, but 1.4 and 1.3 fold increases in protein expression of CYP27A1 and CYP8B I, respectively. V. album caused 9, 1.3 and 2.4 fold decrease in mRNA expression of CYP7A1, CYP27A1, CYP7B1, respectively, however, 4.2 fold increase in mRNA expression of CYP8B1. Injection of o-coumaric acid to rats resulted 0.8 fold decrease in protein expression of CYP7A1, CYP27A1, CYP8B1 and CYP7B1. However, o-coumaric acid caused 1.8, 1.3, 2.3 and 2.3 fold increase in mRNA expression of CYP7A1, CYP27A1, CYP8B 1 and CYP7B1, respectively. According to results, even though V. album and o-coumaric acid caused fold change in protein and mRNA expressions of bile acid metabolizing CYPs, serum cholesterol levels remained unchanged. Thus, the idea can be taken about the role of this plant and its major polyphenolic ingredient in drug discovery and development.

Subject Keywords

Viscum album L., O-coumaric acid, Bile acids, CYP450s, Medicinal plants

URI

https://hdl.handle.net/11511/55640

Journal

FRESENIUS ENVIRONMENTAL BULLETIN

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Determination of phenolic compound profiles and antioxidant effect of black elderberry, echinacea and iceland moss extracts on late-release soft lozenge Yıldız, Özge; Bayındırlı, Alev; Çapanoğlu Güven, Esra; Department of Food Engineering (2021-6) Medicinal plants contain different bioactive compounds that have immune system stimulant, anti-inflammatory, antibacterial, antiviral, antifungal, anticancer and wound healing effects. Echinacea purpurea L., Sambucus nigra L. and Cetraria islandica L. are known with their high antioxidant content and health promoting properties. The main objective of the study was to determine the total phenolic content, total flavonoid content and antioxidant capacity of each plant extract with water and 75 % ethanol as so...
STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING SUNGUR, S; ELCIN, YM; Akbulut, Ural (Elsevier BV, 1992-01-01) Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).
Quantitative expression analysis of the genes potentially involved in clavulanic acid overproduction in streptomyces clavuligerus Aktaş, Caner; Özcengiz, Gülay; Department of Molecular Biology and Genetics (2018) Streptomyces clavuligerus is the producer of the medically important β-lactam antibiotics, including cephamycin C (CC) and the potent β-lactamase inhibitor clavulanic acid. (CA). We have already undertaken an extensive comparative proteomic analysis by using both 2-DE-MALDI-MS and GeLC-MS approaches between an industrial CA overproducer, namely DEPA and the reference strain NRRL3585. In this context, we documented several differentially expressed (over- and under-represented) proteins accounting for high CA...
Estimation of lipid properties related to supercritical fluid extraction Yener, Meryem Esra (2001-01-01) The correlations for vapor pressures of fatty acids, fatty acid methyl and ethyl esters, and simple triglycerides were given based on the available data in the literature. The developed equations enable easy estimation of the critical properties. The group contribution methods were used to develop the estimation method. The estimated properties of the most common lipids were tabulated and the differences between the estimation methods were critically discussed.
Synthesis of TPA impregnated SBA-15 catalysts and their performance in polyethylene degradation reaction Aydemir, Bugce; Sezgi, Naime Aslı; Doğu, Timur (2012-08-01) Tungstophosphoric acid (TPA)-containing mesoporous santa barbara amorphous (SBA)-15 materials were synthesized by impregnation of TPA into hydrothermally synthesized SBA-15. TPA was incorporated to the porous framework of silica with different W/Si ratios, using TPA hydrate as the acid source. The synthesized materials had a surface area range of 212-825 m2 g-1, depending on the TPA loading and exhibited Type IV adsorptiondesorption isotherms. Energy dispersive spectrometry and X-ray photoelectron spectrosc...

Citation Formats

T. Ege, A. M. G. Ozkan, A. Sena, and O. Adalı, “EVALUATION OF IN VIVO EFFECTS OF VISCUM ALBUM L. AND ITS CONSTITUENT O-COUMARIC ACID ON BILE ACID SYNTHESIZING CYPS,” FRESENIUS ENVIRONMENTAL BULLETIN, pp. 2825–2834, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55640.