Ege, Tuba
Ozkan, Ayse Mine Gencler
Sena, Alaattin
Adalı, Orhan
This research was designed to determine about the possible potency of medicinal plants Viscum album L. and its major polyphenolic ingredient, o-coumaric acid on rat liver cholesterol and bile acid metabolizing enzymes. Bioactive compounds of V. album extracts were identified using LC-MS technique. The water extracts of V. album (10 mg/kg) and o-coumaric acid (30 mg/kg) were injected intraperitoneally for 9 days. Then, in vivo effects of this plant and its major phenolic ingredient (o-coumaric acid) on rat liver cholesterol and bile acid metabolizing cytochromes P450s were analyzed by determining protein and mRNA expression levels using western blotting and qRT-PCR techniques, respectively. The results showed that V. album caused a 0.9 fold decrease in protein expression of CYP7A1 and CYP7B1, but 1.4 and 1.3 fold increases in protein expression of CYP27A1 and CYP8B I, respectively. V. album caused 9, 1.3 and 2.4 fold decrease in mRNA expression of CYP7A1, CYP27A1, CYP7B1, respectively, however, 4.2 fold increase in mRNA expression of CYP8B1. Injection of o-coumaric acid to rats resulted 0.8 fold decrease in protein expression of CYP7A1, CYP27A1, CYP8B1 and CYP7B1. However, o-coumaric acid caused 1.8, 1.3, 2.3 and 2.3 fold increase in mRNA expression of CYP7A1, CYP27A1, CYP8B 1 and CYP7B1, respectively. According to results, even though V. album and o-coumaric acid caused fold change in protein and mRNA expressions of bile acid metabolizing CYPs, serum cholesterol levels remained unchanged. Thus, the idea can be taken about the role of this plant and its major polyphenolic ingredient in drug discovery and development.


Determination of phenolic compound profiles and antioxidant effect of black elderberry, echinacea and iceland moss extracts on late-release soft lozenge
Yıldız, Özge; Bayındırlı, Alev; Çapanoğlu Güven, Esra; Department of Food Engineering (2021-6)
Medicinal plants contain different bioactive compounds that have immune system stimulant, anti-inflammatory, antibacterial, antiviral, antifungal, anticancer and wound healing effects. Echinacea purpurea L., Sambucus nigra L. and Cetraria islandica L. are known with their high antioxidant content and health promoting properties. The main objective of the study was to determine the total phenolic content, total flavonoid content and antioxidant capacity of each plant extract with water and 75 % ethanol as so...
SUNGUR, S; ELCIN, YM; Akbulut, Ural (Elsevier BV, 1992-01-01)
Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).
Estimation of lipid properties related to supercritical fluid extraction
Yener, Meryem Esra (2001-01-01)
The correlations for vapor pressures of fatty acids, fatty acid methyl and ethyl esters, and simple triglycerides were given based on the available data in the literature. The developed equations enable easy estimation of the critical properties. The group contribution methods were used to develop the estimation method. The estimated properties of the most common lipids were tabulated and the differences between the estimation methods were critically discussed.
Investigation of the effect of sodium butyrate induced differentiation on inflammatory pathways in colon cancer cells
Küçükdemir, Mümine; Banerjee, Sreeparna; Department of Biology (2012)
Sodium butyrate (NaBt) is a four-carbon short chain fatty acid, produced naturally in colon as the end product of the bacterial anaerobic metabolism on dietary fibers. It was previously shown that NaBt can induce differentiation and may inhibit proliferation. The objective of this study was to investigate the effect of NaBt-induced differentation on inflammatory pathways in HT29 colon cancer cells. For this purpose, first, cells were treated with varying concentrations of NaBt from 1-5 mM and amount require...
Investigation of amino acid modifications derived from lipid oxidation in foods
Karademir, Yeşi; Öztop, Halil Mecit; Department of Food Engineering (2019)
This study aimed to investigate lipid derived amino acid modifications in foods. When the revealed carbonyl pool resulted from the repeated use of frying oils and high absorption rates of the oil during frying are taken into consideration, deep-fat fried foods have a significant potential to investigate this type of reactions. In the first part, effect of heating time of the frying oil on amino acid modifications in potato chips and model doughs was investigated. 2,4-decadienal, total free amino acid, decad...
Citation Formats
T. Ege, A. M. G. Ozkan, A. Sena, and O. Adalı, “EVALUATION OF IN VIVO EFFECTS OF VISCUM ALBUM L. AND ITS CONSTITUENT O-COUMARIC ACID ON BILE ACID SYNTHESIZING CYPS,” FRESENIUS ENVIRONMENTAL BULLETIN, pp. 2825–2834, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/55640.