Design of an emission ratiometric biosensor from MerR family proteins: A sensitive and selective sensor for Hg(II)

Wegner, Seraphine V.
Okesli, Ayse
Chen, Peng
He, Chuan
A ratiometric fluorescent biosensor for Hg2+ is constructed from the MerR protein and a duplex DNA containing a pyrene excimer.


Assessment of machine-learning- versus experimental structural models of sex-specific isoforms of linker histone H1
Uça, Merve; Dimitrov, Stefan; Kale, Seyit (Orta Doğu Teknik Üniversitesi Enformatik Enstitüsü; 2022-10)
The nucleosome is the repeating unit of chromatin and it is composed of an octamer histone core that holds together around 145 base pairs of DNA in a disc-shaped configuration. In higher eukaryotes, an additional histone, known as linker histone H1, provides further structural order by stabilizing the nucleosomal DNA termini. Histone H1 family includes seven somatic variants (H1.0 to H1.5 and H1.10) and four variants expressed only in germ cells, three of which are testisspecific (H1T, H1T2m and HILS1), and...
Fabrication of a Novel Polymeric Scaffold for Amperometric Laccase Biosensor
Soylemez, Saniye; Bekmezci, Aybüke; Göker, Seza; Toppare, Levent Kamil (Wiley, 2019-11-14)
Present work displays the preparation of an electrochemical biosensor using a conjugated polymer and laccase enzyme for catechol quantification in samples. The biosensing system is based on an enzyme immobilization on polymer modified graphite transducer surface. For that purpose, a random conjugated polymer, thienothiophene-benzoxadiazole-alt-benzodithiophene (BOTT), was coated onto a graphite electrode surface via drop casting method followed by immobilization of a biomolecule (laccase) for sensing experi...
Realization of polarization-angle-independent fishnet-based waveguide metamaterial comprised of octagon shaped resonators with sensor and absorber applications
Sabah, Cumali (Springer Science and Business Media LLC, 2016-05-01)
A new fishnet-based waveguide metamaterial structure for the microwave region is introduced and investigated both numerically and experimentally. The proposed model is designed and fabricated on both sides of the substrate and exhibits strong metamaterial behavior (such as negative material parameters: i.e. negative permittivity, negative permeability, and negative index of refraction) at the resonance. Only one single slab is used in the simulation and experiment which provides a reduction in the number of...
Design of a sequence based miRNA clustering method; analysis of fungal miRNAs and host organism target genes
Narcı, Kübra; Akkaya, Mahinur S.; Oğul, Hasan; Department of Bioinformatics (2014)
MicroRNAs are small non-coding RNA molecules which contain 21-25 nucleotides, and function in post transcriptional regulation by inhibiting the translation of mRNA targets. miRNAs typically affect gene regulation by forming composite feed forward circuits (cFFCs) which also comprise a transcription factor (TF) and a target gene. By analyzing these cFFCs, the contribution of miRNAs in altering TF networks can be revealed. These contributions could either be the de-escalation of the target gene repertoire or ...
Preparation of silica coated cobalt ferrite magnetic nanoparticles for the purification of histidine-tagged proteins
Aygar, Gulfem; Kaya, Murat; Özkan, Necati; Kocabıyık, Semra; Volkan, Mürvet (2015-12-01)
Surface modified cobalt ferrite (CoFe2O4) nanoparticles containing Ni-NTA affinity group were synthesized and used for the separation of histidine tag proteins from the complex matrices through the use of imidazole side chains of histidine molecules. Firstly, CoFe2O4 nanoparticles with a narrow size distribution were prepared in an aqueous solution using the controlled co-precipitation method. In order to obtain small CoFe2O4 agglomerates, oleic acid and sodium chloride were used as dispersants. The CoFe2O4...
Citation Formats
S. V. Wegner, A. Okesli, P. Chen, and C. He, “Design of an emission ratiometric biosensor from MerR family proteins: A sensitive and selective sensor for Hg(II),” 2007, vol. 233, p. 430, Accessed: 00, 2020. [Online]. Available: