Natural origin silica reinforced dual fiber matrices for bone tissue engineering

Download

12625739.pdf

Date

2020-10

Author

Dalgıç, Ali Deniz

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

232
views

164
downloads

Graft therapy is used to treat bone tissue loss, which has drawbacks; donor scarcity, risk of disease transmission and immune reaction. Tissue engineering scaffolds can overcome these drawbacks. In this study, a 3D scaffold that will support tissue regeneration at defect site was developed using mainly natural materials. Scaffold was produced by co-electrospinning and had two distinct fiber phases; first fiber phase was produced from a bacterial origin polymer, Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and small amount of Polycaprolactone (PCL) to support fiber structure, second fiber phase was formed by pullulan (PUL) polymer and diatom silica shells (DS) from single cell algae origin. Also, in the study, PHBV production by Cupriavidus necator bacterial strain was optimized and used to produce scaffold groups. PHBV/PCL/CA:PUL/DS scaffold group was produced as dual fiber matrix and a new crosslinking method for PUL was developed for crosslinking through electrospinning. Cefuroxime Axetil (CA) antibiotic was loaded into hydrophobic PHBV fibers to sustain antibiotic release from scaffold. Characterization studies revealed that, in aqueous environment scaffold degrade slowly, take less water and has stable structure, support controlled release of antibiotic and improved compressive strength due to incorporated DS and double fiber structure. In vitro studies revealed that DS bearing groups improved Saos-2 cell viability and co-electrospun groups that have hydrophilic PUL fibers supported L929 cell viability. Scanning electrone microscopy and confocal laser scanning microscopy analyses showed that cells distributed through co-electrospun scaffold with healthy morphology. In the study, a novel, 3D, dual fiber scaffold was produced with natural origin base materials and has potential to be used for bone tissue engineering applications.

Subject Keywords

Bone Tissue Engineering, Diatom, Co-Electrospinning, Pullulan, Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate)

URI

https://hdl.handle.net/11511/69273

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Natural origin bilayer pullulan-PHBV scaffold for wound healing applications Dalgic, Ali Deniz; Koman, Ezgi; Karataş, Ayten; Tezcaner, Ayşen; Keskin, Dilek (2022-03-01) Skin tissue loss that occurs by injury and diseases can turn into chronic wounds as a result of complications alongside infection. Chronic wounds fail to heal by themselves and need advanced treatments like engineered wound dressings and regenerative scaffolds. In this study, a novel, natural origin, bilayer electrospun scaffold was produced from pullulan (PUL) and poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) biopolymers. PHBV production by Cupriavidus necator bacterial strain was optimized and produced ...
Simvastatin loaded porous hydroxyapatite based microcarriers for bone tissue engineering / Güldiken, Merve; Tezcaner, Ayşen; Durucan, Caner; Department of Biotechnology (2014) Bone tissue engineering provides a new medical therapy as an alternative to conventional bone replacement grafts. Carriers designed for bone tissue engineering applications should be biocompatible, bioactive, and porous and should also meet certain minimal requirements to obtain functional engineered tissues. Polymers, ceramic materials and their composites are widely used for developing such carriers. The objective of this study was to develop and characterize a simvastatin (SIM) loaded porous hydroxyapati...
Preparation of Barium doped Baghdadite/PHBV fibrous scaffolds for bone tissue engineering Sadreddini, Sanaossadat; Evis, Zafer; Keskin, Dilek; Department of Biomedical Engineering (2023-1-23) Recently, bioceramic/polymer composites have dragged a lot of attention for treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop fibrous composite poly(hydroxybutyrate) co (hydroxyvalerate)- polycaprolactone, PHBV-PCL, scaffolds containing different amounts of baghdadite (BAG) and Ba-doped BAG that can provide bone regeneration in the bone defect area and to investigate the effect of these scaffolds on the structural, mechanical, and biological propertie...
3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering Yedekçi, Buşra; Evis, Zafer; Tezcaner, Ayşen; Department of Engineering Sciences (2021-9-6) Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped hydroxyapatite (HA) that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HAs were s...
Synthesis of baghdadite using modified sol–gel route and investigation of its properties for bone treatment applications Jodati, Hossein; Tezcaner, Ayşen; Evis, Zafer; Alshemary, Ammar Z; ÇELİK, ERDAL (2022-01-01) The requirement for biomaterials with superior properties, used in bone treatment applications, is inevitable due to escalated bone tissue defects. Baghdadite (BAG) is a calcium silicate that benefits from the presence of zirconium (Zr) in its structure and has attracted huge attention in recent years. In this study, a modified sol–gel route was proposed to synthesize BAG by dissolving Zr precursor separately and using optimum amounts of solvent and chelating agent. Due to thermal gravimetric analysis and d...

Citation Formats

A. D. Dalgıç, “Natural origin silica reinforced dual fiber matrices for bone tissue engineering,” Ph.D. - Doctoral Program, Middle East Technical University, 2020.