Magnesium Oxide Nanocomposites for Improving Orthopedic Tissue Regeneration

Date

2014-12-31

Author

Hıckey, Danıel
Sun, Lınlın
Ercan, Batur
Webster, Thomas

Metadata

Show full item record

Item Usage Stats

165
views

0
downloads

Where ligaments meet bone, there exists a transitional region of heterogeneous tissue that is graded from hard, highly mineralized fibrocartilage at the bone interface to un-mineralized soft tissue at the ligament. This structure, called the enthesis, disperses stress concentrations that arise due to the vastly different mechanical properties of bone and ligaments. However, in the event that a ligament is injured and requires surgery, the tendon-to-bone insertion site (TBI) must be destroyed and is not easily restored due to avascularity in the region and because of slow and disorganized healing within heterogeneous tissues. It is believed that this loss of enthesis functionality following joint reconstructive surgery is a leading cause of high failure rates for such surgeries (5-25% failure rates for ACL surgery) [1]. Therefore, there is considerable interest in the development of a nanostructured biomaterial that is capable of regenerating the TBI. In this study, magnesium oxide (MgO) nanoparticles were used to mineralize poly(l-lactic acid) (PLLA) and tested for their ability to improve the attachment and growth of TBI-related orthopedic tissue. Magnesium is an essential mineral in bone which is thought to regulate the size and density of hydroxyapatite (HA) crystals, and further, Weng and Webster demonstrated that nano-rough MgO increased bone cell density three-fold compared to bulk MgO [2]. Presently, the ability of these materials to promote tissue growth at the TBI was characterized via cell adhesion and proliferation experiments with fibroblasts and osteoblasts. Materials were also tested for their mechanical properties, and further characterization was performed using SEM, TEM, XRD, FTIR, EDS, and contact angle tests.

URI

https://hdl.handle.net/11511/85832

Conference Name

Society For Biomaterials 2014 Annual Meeting and Exposition, (16 - 19 Nisan 2014)

Collections

Department of Metallurgical and Materials Engineering, Conference / Seminar

Suggestions

OpenMETU
Core

Composite clinoptilolite/PCL-PEG-PCL scaffolds for bone regeneration: In vitro and in vivo evaluation Pazarçeviren, Ahmet Engin; Altunbas, Korhan; Yaprakci, Volkan; Erdemli, Ozge; Keskin, Dilek; Tezcaner, Ayşen (Wiley, 2020-01-01) In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time. Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compression molding technique with varying CLN contents. We hypothesized that CLN reinforcement in a composite scaffold will improve bone regeneration and promote repair. Therefore, the scaffolds we...
Graphene Oxide Incorporated Silicate Doped Nano-hydroxyapatite Novel Composite for Bone Tissue Engineering Dalgıç, Ali Deniz; Tezcaner, Ayşen; Keskin, Dilek; Evis, Zafer (2018-06-05) Hydroxyapatite, as the natural inorganic phase of the bone tissue has been widely used as the main structure of bone tissue scaffolds. In studies, ion substitution into hydroxyapatite structure enhanced the osteogenic properties. Silicon is an ion that was used to produce silicate doped hydroxyapatite (SiHA). SiHA is reported to promote proliferation and osteogenic activity of osteoblasts. Graphene oxide (GO) is formed by functionalization of graphene with oxygen-containing groups, reported to enhance adhes...
MgO nanocomposites as new antibacterial materials for orthopedic tissue engineering applications Hickey, Daniel J.; Ercan, Batur; Chung, Stanley; Ercan, Batur; Sun, Linlin; Geilich, Ben (2014-01-01) Regeneration of orthopedic soft and hard tissues, such as ligaments, bone, and the tendon-to-bone insertion site (TBI), is problematic due to a lack of suitable biomaterials which possess appropriate mechanical properties capable of promoting cellular functions in these tissues with limited regenerative capacity. Additionally, surgically implanted biomaterials are susceptible to bacterial infection, which can lead to implant failure, as well as further complications such as wide-spread infection. To address...
Skeletal muscle patch engineering on synthetic and acellular human skeletal muscle originated scaffolds Ay, Birol; KARAÖZ, Erdal; KESEMENLİ, CUMHUR CEVDET; KENAR, HALİME (2017-03-01) The reconstruction of skeletal muscle tissue is currently performed by transplanting a muscle tissue graft from local or distant sites of the patient's body, but this practice leads to donor site morbidity in case of large defects. With the aim of providing an alternative treatment approach, skeletal muscle tissue formation potential of human myoblasts and human menstrual blood derived mesenchymal stem cells (hMB-MSCs) on synthetic [poly(l-lactide-co-caprolactone), 70:30] scaffolds with oriented microfibers...
Collagen-based meniscus tissue engineering: design and application Halili Ndreu, Albana; Hasırcı, Nesrin; Hasırcı, Vasıf Nejat; Department of Biotechnology (2011) Meniscus is a wedge shaped structure, with a convex base attached to a flat tibial surface, and with a concave femoral surface, on which femur and tibia articulate. It has several functions including joint lubrication, shock absorption, load transmission and joint stability. Various methods were tried to treat meniscal tears but each has its own drawbacks. Tissue engineering seems to be a promising solution that avoids all the problems associated with the other approaches. In this study, a three dimensional...

Citation Formats

D. Hıckey, L. Sun, B. Ercan, and T. Webster, “Magnesium Oxide Nanocomposites for Improving Orthopedic Tissue Regeneration,” presented at the Society For Biomaterials 2014 Annual Meeting and Exposition, (16 - 19 Nisan 2014), Northeastern University, Boston, MA, USA, 2014, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/85832.