ANDROID BASED PORTABLE CELL COUNTING SYSTEM FOR LABEL FREE QUANTIFICATION OF DEP MANIPULATED CANCER CELLS

Date

2017-06-22

Author

Aslan, Mahmut Kamil
Külah, Haluk

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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This paper presents a portable system integrating microfluidic dielectrophoresis (DEP) channel with a CMOS imager for label and lens free detection, and real-time counting of MCF-7 breast cancer cells. The DEP device is designed for trapping the MCF-7 that can simultaneously be imaged using a CMOS sensor. An android application is employed in order to record raw CMOS images. Cells are detected and counted using inbuilt image processing operations of the same application. Average counting accuracy of the system is reported as 90%. Additionally, the whole system is powered with the cell phone battery eliminating the need for an external power supply.

Subject Keywords

Lab-on-a-chip (LOC), Android, Mobile, Dielectrophoresis (DEP), CMOS imaging, Label-free, MCF-7, Fluorescence microscopy

URI

https://hdl.handle.net/11511/52820

Conference Name

19th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers)

Collections

Department of Electrical and Electronics Engineering, Conference / Seminar

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LABEL-FREE DETECTION OF LEUKEMIA CELLS WITH A LAB-ON-A-CHIP SYSTEM INTEGRATING DIELECTROPHORESIS AND CMOS IMAGING Demircan, Y.; Orguc, S.; Musayev, J.; Ozgur, E.; Erdem, Murat; Gündüz, Ufuk; Eminoglu, S.; Külah, Haluk; Akın, Tayfun (2015-06-25) This paper presents a fully-integrated lab-on-a-chip (LOC) system for label-free detection and real-time counting of dielectrophoretically trapped multidrug resistant (MDR) K562 cells. The system integrates a parylene-based microfluidic DEP channel on top of a CMOS image sensor for the first time in the literature. The DEP channel can trap MDR K562 cells with 9 V-pp and 10 mu l/min flow rate, and the CMOS image sensor can detect the trapped cells as small as 3 mu m in diameter with a noise level of 28.3 e(-...
Dielectrophoretic detection of imatinib resistance in K562 Cells Using A lab-on-a-chip system Demircan Yalçın, Yağmur; Toral, Taylan Berkın; Sukaş, Serkan; Yıldırım, Ender; Zorlu, Özge; Gündüz, Ufuk; Külah, Haluk (Chemical and Biological Microsystems Society (CBMS); 2019-10-27) This study presents label-free detection of imatinib resistance in K562 cells via integrated dielectrophoretic detection and impedimetric counting on a lab-on-a-chip system. Two impedimetric counting units were placed upstream and downstream of dielectrophoretic detection unit to obtain differential cell count and consequently the trapping ratio. The trapping ratio up to 57% was achieved for imatinib resistant (~60-fold) K562 cells, while it was minimized to 20% for wild type K562 cells
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Citation Formats

M. K. Aslan and H. Külah, “ANDROID BASED PORTABLE CELL COUNTING SYSTEM FOR LABEL FREE QUANTIFICATION OF DEP MANIPULATED CANCER CELLS,” presented at the 19th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers), Kaohsiung, TAIWAN, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/52820.