Immobilization of invertase and a-amylase using different support systems by chemical crosslinking

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1993

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Emregül(Aysu), Emel

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https://hdl.handle.net/11511/8920

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Graduate School of Natural and Applied Sciences, Thesis

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Immobilization of Invertase in a Novel Proton Conducting Poly(vinylphosphonic acid) - poly(1-vinylimidazole) Network Isikli, Suheda; Tuncagil, Sevinc; Bozkurt, Ayhan; Toppare, Levent Kamil (Informa UK Limited, 2010-01-01) A novel proton conducting polymer blend was prepared by mixing poly(vinylphosphonic acid) (PVPA) with poly(1-vinylimidazole) (PVI) at various stoichiometric ratios via changing molar ratio of monomer repeating unit to achieve the highest protonation. The polymer network having the most suitable stoichiometric ratio for substantial proton conductivity was prepared and characterized by FT-IR spectroscopy and proton conductivity measurements. The network was used for immobilization of invertase and some import...
Immobilization of Invertase in DAD Type Polymers: Combination of Benzothiadiazole Acceptor Unit and 3,4-Ethylenedioxythiophene and Thiophene Donor Units Aydar, Sevda; BÖYÜKBAYRAM, AYŞE ELİF; Sendur, Merve; Toppare, Levent Kamil (Informa UK Limited, 2011-01-01) Conductive polymers with donor-acceptor-donor (DAD) type units; benzothiadiazole acceptor unit and 3,4-ethylenedioxythiophene (EDOT) and thiophene (Th) donor units, were investigated for immobilization of invertase. The polymers were prepared potentio-dynamically from their monomers, M-1 (Th-benzothiadiazole-Th), M-2 (EDOT-benzothiadiazole-EDOT), M-3 (Th-benzothiadiazoleEDOT) and a copolymer, which is a homolog to homopolymer of M-3, was prepared from M-1 and M-2. Invertase was trapped between a two layer-c...
Immobilization of invertase and glucose oxidase in conducting H-type polysiloxane/polypyrrole block copolymers Gursel, A; Alkan, S; Toppare, Levent Kamil; Yagci, Y (Elsevier BV, 2003-01-01) In this study, immobilizations of enzymes, invertase and glucose oxidase, were achieved in conducting copolymers of N-pyrrolyl terminated polydimethylsiloxane/polypyrrole (PDMS/PPy) matrices via electrochemical polymerization. The kinetic parameters, v(max) (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on enzyme activity and film morphologies was examined. ...
Immobilization of invertase and glucose oxidase in poly 2-(3-thienyl) acetate/polypyrrole matrices Işık, Sonnur; Toppare, Levent; Department of Chemistry (2002)
Immobilization of invertase and glucose oxidase in poly 2-methylbutyl-2-(3-thienyl) acetate/polypyrrole matrices Isik, S; Alkan, S; Toppare, Levent Kamil; Cianga, I; Yagci, Y (Elsevier BV, 2003-12-01) Immobilization of invertase and glucose oxidase in conducting polypyrrole and copolymers of poly 2-methylbutyl-2-(3-thienyl) acetate with pyrrole were achieved via electrochemical method. Sodium dodecyl sulphate was found to be the most suitable supporting electrolyte. Maximum reaction rate, Michaelis-Menten constant and optimum temperatures were determined for native and immobilized enzymes. Storage and operational stabilities of enzyme electrodes were also investigated.

Citation Formats

E. Emregül(Aysu), “Immobilization of invertase and a-amylase using different support systems by chemical crosslinking,” Middle East Technical University, 1993.