Effects of Medicinal Plant Sanguisorba minor Subsp. balearica on Selected CYP450 Isozymes and Proinflammatory Cytokines Activity in Inflammation Induced Rat Model: An Immunochemical Approach

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2021-7-01
inan Genç, Aysun
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Medicinal uses of plant extracts have been based on traditional knowledge without enough scientific evidence. Sanguisorba minor subsp. balearica was used traditionally to treat various diseases. This study was performed to evaluate the antiinflammatory effect of Smb. Experimental groups are; healthy control, CFA control, diclofenac sodium, quercetin, and 34-70mg/kg Smb treated groups. Antiinflammatory effect was examined on Complete Freund’s Adjuvant model, using biochemical, immunohistopathological, immunohistochemical, and immunoblotting methods on the CYP1A2, CYP3A4, CYP2C9, CYP2E1 enzymes as well as TNF-α, IL-1 beta, and IL-12. CFA group shows an increase in both TNF-α and IL-1beta. The remaining groups show a decrease in both cytokines. CFA group exhibited lots of inflammatory signs, diclofenac sodium has a recovery effect on inflammation. 34 and 70 mg/kg Smb and quercetin ameliorated the inflammation. CFA group has the highest TNF-α and IL-12. 34-70mg/kg Smb and diclofenac sodium groups have lower TNF-α and IL-12. Quercetin group has the lowest TNF-α and IL-12. In all treated groups, CYP1A2 levels were lower than the healthy controls. CYP2C9 levels in the positive control and 34-70mg/kg Smb groups were higher than the healthy controls. CYP2C9 levels in negative control and quercetin groups were lower levels. CYP3A4 and CYP2E1 levels in all treated groups were higher than in the healthy controls. There isn’t enough data to clarify the possible effects of Smb on CYP450s and their relation with the anti-inflammatory response. This current study aims to clarify the possible anti-inflammatory effects of Smb on proinflammatory cytokines and on targeted CYP450 on the CFA model.
Sanguisorba minor subsp. balearica, CYP450, Cytokines, Inflammation, Complete Freund’s Adjuvant model
https://hdl.handle.net/11511/91461
Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats
A. inan Genç, “Effects of Medicinal Plant Sanguisorba minor Subsp. balearica on Selected CYP450 Isozymes and Proinflammatory Cytokines Activity in Inflammation Induced Rat Model: An Immunochemical Approach,” Ph.D. - Doctoral Program, Middle East Technical University, 2021.
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