Effects of Medicinal Plant Sanguisorba minor Subsp. balearica on Selected CYP450 Isozymes and Proinflammatory Cytokines Activity in Inflammation Induced Rat Model: An Immunochemical Approach

inan Genç, Aysun
Medicinal uses of plant extracts have been based on traditional knowledge without enough scientific evidence. Sanguisorba minor subsp. balearica was used traditionally to treat various diseases. This study was performed to evaluate the antiinflammatory effect of Smb. Experimental groups are; healthy control, CFA control, diclofenac sodium, quercetin, and 34-70mg/kg Smb treated groups. Antiinflammatory effect was examined on Complete Freund’s Adjuvant model, using biochemical, immunohistopathological, immunohistochemical, and immunoblotting methods on the CYP1A2, CYP3A4, CYP2C9, CYP2E1 enzymes as well as TNF-α, IL-1 beta, and IL-12. CFA group shows an increase in both TNF-α and IL-1beta. The remaining groups show a decrease in both cytokines. CFA group exhibited lots of inflammatory signs, diclofenac sodium has a recovery effect on inflammation. 34 and 70 mg/kg Smb and quercetin ameliorated the inflammation. CFA group has the highest TNF-α and IL-12. 34-70mg/kg Smb and diclofenac sodium groups have lower TNF-α and IL-12. Quercetin group has the lowest TNF-α and IL-12. In all treated groups, CYP1A2 levels were lower than the healthy controls. CYP2C9 levels in the positive control and 34-70mg/kg Smb groups were higher than the healthy controls. CYP2C9 levels in negative control and quercetin groups were lower levels. CYP3A4 and CYP2E1 levels in all treated groups were higher than in the healthy controls. There isn’t enough data to clarify the possible effects of Smb on CYP450s and their relation with the anti-inflammatory response. This current study aims to clarify the possible anti-inflammatory effects of Smb on proinflammatory cytokines and on targeted CYP450 on the CFA model.


Effects of Water Soluble Garlic Extract on Human Leukemia HL60 Cell Lines
Purpose: Studies with garlic have shown the medicinal value of the plant by its antioxidant potential which is exerted by its organosulfur rich content. In this study, the aim is to determine any potential antioxidant efficacy of the less toxic water soluble extract of a widely cultivated garlic form in Turkey, in terms of alterations on the oxidant/antioxidant status of HL60 promyelocytic leukemia cell line.
A comparative study on the phenolic composition, antioxidant and enzyme inhibition activities of two endemic Onosma species
Saravanakumar, Kandasamy; Sarikurkcu, Cengiz; Sarikurkcu, Rifat Tayyib; Wang, Myeong-Hyeon (Elsevier BV, 2019-12-15)
The studies on bioactivities of plant extracts are a fundamental requirement for future pharmacological research. Therefore, the present study extracted the phytochemicals from the two endemic species such as Onosma isaurica and O. bracteosa and tested their antioxidant, reducing power and enzyme inhibitory activities followed by scanning of the phytochemicals in the extracts by using liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS). The results revealed that the antioxidant activi...
Optimization of hypericin extraction from hypericum perforatum L. tissues and evaluation of its applicability in dye-sensitized solar cells /
Aktuna, Özge; Öktem, Hüseyin Avni; Eyidoğan, Füsun İnci; Department of Biotechnology (2014)
Medicinal plants which have been widely used in folk medicine are known to contain important biologically active compounds. Hypericum perforatum is one of the medicinal plants that grows in Europe, Western Asia and Northern Africa and is distinguished by its golden yellow flowers. The common name of the plant is St. John’s wort. As a practical folk-remedy, it has been used widely to heal wounds, remedy kidney troubles, and alleviate nervous disorders. Nowadays, many application areas of hypericin are discov...
Ege, Tuba; Ozkan, Ayse Mine Gencler; Sena, Alaattin; Adalı, Orhan (2019-01-01)
This research was designed to determine about the possible potency of medicinal plants Viscum album L. and its major polyphenolic ingredient, o-coumaric acid on rat liver cholesterol and bile acid metabolizing enzymes. Bioactive compounds of V. album extracts were identified using LC-MS technique. The water extracts of V. album (10 mg/kg) and o-coumaric acid (30 mg/kg) were injected intraperitoneally for 9 days. Then, in vivo effects of this plant and its major phenolic ingredient (o-coumaric acid) on rat l...
Antioxidant and cytotoxic properties of Salvia fruticosa M. and its effects on gene expressions of some CYP450 and antioxidant enzymes in HT-29 cell line
Altay, Ahmet; Bozoğlu, Faruk; Tokuşoğlu, Özlem; Department of Biochemistry (2015)
Salvia genus is a widely cultivated as a perennial herb and used in medicine for various purposes due to its antimicrobial, antioxidant, anticarcinogen and anti-inflammatory properties. In this study, the phenolic composition of water extract of Salvia fruticosa and possible effects of those constituents in cancer related drug metabolizing enzymes are investigated. S. fruticosa extract displayed high Radical Scavenging Activity against DPPH and ABTS radicals and also showed high Fe2+ Chelating Activity . To...
Citation Formats
A. inan Genç, “Effects of Medicinal Plant Sanguisorba minor Subsp. balearica on Selected CYP450 Isozymes and Proinflammatory Cytokines Activity in Inflammation Induced Rat Model: An Immunochemical Approach,” Ph.D. - Doctoral Program, Middle East Technical University, 2021.