Studies Towards the Preparation of Functional Cellulose Microspheres with an Affinity Towards Bacterial Cells

Date

2021-04-08

Author

Demirel, Kaan
Akbulut, Doğan
Yılmaz, Ozan
Türkyılmaz, Serhan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

205
views

0
downloads

Cellulose microspheres (CMs, diameters 10-1000 m) have been used as stationary phases in chromatography, substrates for ion exchange applications, supports for protein immobilization and solid-phase synthesis, and drug loading and release materials.2 Furthermore CMs are biocompatible and have been used in many biomedical applications as varied as blood filtration3,4 and the dressing of wounds. Blood is normally sterile and the presence of bacteria in blood is a condition known as bacteremia. Bacteremia can be caused by burns, injuries, local infections, surgical procedures, and the use of catheters or needles. If undetected and untreated, the immune response to bacteremia can result in sepsis, septic shock, and multiple organ failure. Septic shock and multiple organ failure as a result of hospital-acquired bacteremia is a leading cause of death in intensive care units.5 Zinc(II) bisdipicolylamine complexes (Zn2BDPA, Figure 1, Panel A) are capable of selectively binding to negatively charged phosphate amphiphiles displayed by bacterial cells.6In this study we aimed to develop Zn2BDPA-derivatized CMs with an ultimate goal of obtaining selective sorbents for the treatment of bacteremia through extracorporeal blood filtration. To this end we have investigated the preparation of CMs through the thermal regeneration of cellulose from water-in-oil emulsions of viscose. Native CMs were then activated to display epoxy, amino, and carboxylic acid functionalities. We then tried to couple amine and carboxylic acid reactive BDPA ligands with these activated CMs. Elemental analysis and FTIR spectroscopy confirmed the formation of BDPA-CM conjugates. These Zn2BDPA functionalized CMs were then shown to bind to green fluorescent protein (GFP) expressing bacterial cells through fluorescence microscopy (Figure 1, Panel B and C).Acknowledgement: We gratefully acknowledge financial support from TÜBİTAK (Project 118Z442).Footnotes and References:1) Undergraduate student researcher2) Gericke, M.; Trygg, J.; Fardim P. Chem. Rev. 2013, 113, 4812−4836.3) Wang, S.; Yu, Y. “Bioactive bead type cellulosic adsorbent for blood purification”, 2013 in Cellulose-Medical, Pharmaceutical and Electronic Applications. IntechOpen.4) Weber, V. et al. “Functionalization and application of cellulose microparticles as adsorbents in extracorporeal blood purification.” In: Macromolecular Symposia. Weinheim: WILEY‐VCH Verlag, 2010. p. 90-95.5) Mayr, F.B.; Yende, S., Angus D.C. “Epidemiology of severe sepsis” Virulence 2014, 5(1), 4-11.6) Turkyilmaz, S.; Rice, D.R.; Palumbo, R.; Smith B.D. Org. Biomol. Chem. 2014, 12, 5645-5655.

URI

https://hdl.handle.net/11511/92508

Conference Name

9th International Drug Chemistry Congress

Collections

Department of Chemistry, Conference / Seminar

Suggestions

OpenMETU
Core

Studies towards the preparation of functionalized cellulose microspheres with affinities towards negatively charged biomembranes Demirel, Kaan; Türkyılmaz, Serhan; Department of Chemistry (2021-7) Cellulose microspheres (CMs), generally with diameters in the 10-1000 µm range, have been used as stationary phases in chromatography, substrates for ion exchange applications, supports for protein immobilization and solid-phase synthesis, and drug loading and release materials. Furthermore, cellulose and CMs are biocompatible and have been used in many biomedical applications as varied as the dressing of wounds and blood filtration. Targeting of bacterial cells can be achieved using antibodies, antibody fr...
Speciation of Selenium in Supplements by High Performance Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry BAKIRDERE, Sezgin; Volkan, Mürvet; Ataman, Osman Yavuz (2015-06-13) Anion and cation exchange high-performance liquid chromatography (HPLC) combined with inductively coupled plasma-mass spectrometry (ICP-MS) were used for speciation of selenium in supplements. All the parameters in the extraction, separation, and determination procedures were optimized. Recovery studies for the selenium species from the anion and cation exchange columns were performed and there were no analyte losses. Limits of detection for selenium(IV), selenium(VI), Se in selenomethionine, and Se in sele...
Study on the properties of crosslinking of poly(ethylene oxide) and hydroxyapatite-poly(ethylene oxide) composite Banat, R; Tincer, T (2003-10-10) This study covers the crosslinking of poly-(ethylene oxide) (PEO) and its composite with calcium hydroxyapatite (HA), their mechanical and swelling properties, and morphology. Sheets of the composites of PEO (two different grades with M-v: 5x10(6) and 2x10(5)) and HA and neat PEO were prepared by compression molding. The prepared composite. and. PEO (0.1-mm-thick) sheets were crosslinked with exposure of UV-irradiation in the presence of a photoinitiator, acetophenone (AP). This simple method for crosslinki...
Chemoenzymatic synthesis of chiral hydroxymethyl cycloalkenols Şenocak, Deniz; Demir, Ayhan Sıtkı; Department of Chemistry (2004) Chiral cyclic alkenols with hydroxymethyl functionality are important structural units in many biologically active natural compouds such as prostaglandins, sesquiterpene antiviral agents, pentenomycins, xanthocidin, sarkomycin, etc. 1,3-cycloalkanediones are converted into bicyclic polyoxo derivatives with formaldehyde and trioxane in the presence of Lewis acid. Selective oxidation of the bicyclic compounds by using manganese(III)acetate followed by enzyme-catalyzed kinetic resolution afforded chiral bicycl...
DEVELOPMENT OF 3D PRINTED PECTIN AND GELATIN BASED BIOACTIVE SKIN GRAFTS Bostancı, Nazlı Seray; Tezcaner, Ayşen; Hasırcı, Nesrin; Department of Biotechnology (2021-8-10) The aim of this study was to develop an easily applicable and bioactive skin graft composed of methacrylated pectin (PeMA) and methacrylated gelatin (GelMA), containing curcumin and/or Vitamin-C as antimicrobial and healing accelerating agents, respectively. Grafts were prepared in slab, 3D printed and 3D bioprinted forms and different properties were evaluated. Preparation parameters were optimized using slabs having different compositions (PeMA:GelMA as P:G 1:1, 1:2 and 1:3, v/v). P1:G1 solution did not h...

Citation Formats

K. Demirel, D. Akbulut, O. Yılmaz, and S. Türkyılmaz, “Studies Towards the Preparation of Functional Cellulose Microspheres with an Affinity Towards Bacterial Cells,” presented at the 9th International Drug Chemistry Congress, Antalya, Türkiye, 2021, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/92508.