Salt and nitrogen amendment and optimization for cellulase and xylanase production using dilute acid hydrolysate of distillers' dried grains with solubles (DDGS) as the feedstock

Date

2022-01-01

Author

Iram, Attia
Çekmecelioğlu, Deniz
Demirci, Ali

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

122
views

0
downloads

Distillers' dried grains with solubles (DDGS) is a by-product of dry-mill corn ethanol production comprising a high nutritional value due to residual fiber, protein, and lipid contents. The fiber content of DDGS is high enough to be considered a valuable source for the production of hydrolytic enzymes, such as cellulase and xylanases, which can be used for hydrolysis of lignocellulosic feedstock during ethanol production. The DDGS-based medium prepared after acid hydrolysis provides adequate sugars for enzyme production, while additional macronutrients, such as salts and nitrogen sources, can enhance the enzyme production. Therefore, this study was undertaken to evaluate the effect of salts (KH2PO4, CaCl2 center dot 2H(2)O, MgSO4 center dot 7H(2)O, FeSO4 center dot 7H(2)O, CoCl2 center dot 6H(2)O, and MnSO4 center dot H2O), peptone, and yeast extract on enzyme secretion by four different Aspergillus niger strains and to optimize the nitrogen source for maximum enzyme production. Yeast extract improved the cellulase production (0.38 IU/ml) for A. niger (NRRL 1956) as compared to peptone (0.29 IU/ml). However, maximum cellulase productions of 0.42 IU/ml and 0.45 IU/ml were obtained by A. niger (NRRL 330) and A. niger (NRRL 567), respectively, in presence of ammonium sulfate. The optimized nitrogen amounts resulted in a significant increase in the cellulase production from 0.174 to 0.63 IU/ml on day 9 of the fermentation with A. niger (NRRL 330). The composite model improved both cellulase and xylanase production. In conclusion, the optimization of all three nitrogen sources improved both cellulase and xylanase production in the DDGS-based media.

Subject Keywords

Hydrolytic enzymes, Aspergillus niger, Trichoderma reesei, Media optimization, TRICHODERMA-REESEI RUT-C30, INDUSTRIAL POTATO WASTE, ASPERGILLUS-NIGER, ENZYME-PRODUCTION, AQUEOUS AMMONIA, BIOETHANOL

URI

https://hdl.handle.net/11511/95323

Journal

BIOPROCESS AND BIOSYSTEMS ENGINEERING

DOI

https://doi.org/10.1007/s00449-021-02676-7

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (Elsevier BV, 2019-06-01) Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreat...
Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-10-01) © 2022 Elsevier LtdLignocellulolytic enzymes such as cellulase and xylanases are needed on the industrial scales for low-cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focused on the statistical optimizat...
Bacterial and fungal strain selections for cellulase and xylanase production using distillers' dried grains with solubles (DDGS) Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2020-01-01) Distillers' dried grains with solubles (DDGS) are by-products of dry-mill bioethanol production from corn and wheat. The DDGS is leftover by the yeast after converting the starch into ethanol and it has a high nutritional profile including undigested fiber and protein fractions. The DDGS can be used as cheap microbial feedstock for the production of cellulase and xylanases, which are produced from expensive industrial feedstocks. In this study, screening of several bacterial and fungal strains was done usin...
Optimization of the fermentation parameters to maximize the production of cellulases and xylanases using DDGS as the main feedstock in stirred tank bioreactors Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2022-01-01) Lignocellulolytic enzymes such as cellulases and xylanases are needed on the industrial scales for low cost production of biofuels and the other value-added products from lignocellulosic biomass such as distillers' dried grains with solubles (DDGS). Optimization of fermentation variables such as agitation, aeration, and inoculum size for fungal enzyme production by submerged fermentation can enhance the enzyme production levels. Therefore, this research focuses on the statistical optimization of fungal inoc...
Affinity chromatographic purification of recombinant human growth hormone Balcı, Oğuz; Çalık, Pınar; Department of Biotechnology (2008) The purpose of the study is to purify human growth hormone from the fermentation broth by affinity chromatography. For this purpose, human growth hormone specific oligonucleotide aptamers are selected among an aptamer library; selected oligonucleotides were synthesized and used as ligands. Effect of pH on ligand-human growth hormone complex formation was investigated and the highest complex formation was obtained at pH= 7.0. Human growth hormone is separated from the fermentation broth with 99.8% purity and...

Citation Formats

A. Iram, D. Çekmecelioğlu, and A. Demirci, “Salt and nitrogen amendment and optimization for cellulase and xylanase production using dilute acid hydrolysate of distillers’ dried grains with solubles (DDGS) as the feedstock,” BIOPROCESS AND BIOSYSTEMS ENGINEERING, pp. 0–0, 2022, Accessed: 00, 2022. [Online]. Available: https://hdl.handle.net/11511/95323.