Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
The effects of selenium on stz-induced diabetic rat kidney plasma membrane
Download
index.pdf
Date
2010
Author
Gurbanov, Rafig
Metadata
Show full item record
Item Usage Stats
241
views
64
downloads
Cite This
The kidney is one of the most affected organs of body from diabetes. Diabetic kidney disease is a complication of diabetes seen in 30-40% of diabetic person. The aim of this work is to contribute the useful information in the therapy of diabetes. It is very important to know the role of antioxidants at the molecular level during diabetes. The protecting role of antioxidants against lipid peroxidation, the effect of cellular antioxidant enzyme systems, understanding the changes of membrane fluidity, lipid order and protein structure which are resulted from antioxidant treatment, determining the effective therapeutic dose with the help of biochemical methods are very important in order to understand the effect of antioxidants at molecular level. In this thesis work, the Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) was used in order to study the diabetic kidney disease at the molecular level, which is encountered as a complication of diabetes. Furthermore, the protecting and possible therapeutic role of selenium in the course of diabetic kidney disease was investigated. To conclude, the kidney plasma membranes were severely deteriorated due to diabetes with respect to its lipid, protein and carbohydrate structure and content, which were corrected after selenium treatment. The diabetes causes diminishment of whole membrane fluidity, which was normalized with the selenium administration. This is the first study demonstrating the effect of diabetes on kidney plasma membrane and the effect of selenium on stz-induced diabetic kidney plasma membranes using spectroscopic tools. The study revealed serious therapeutic and preventing capacities of selenium on diabetic kidney plasma membranes which needs confirmation of future researches. Furthermore, the dosage of selenium given to diabetics should be investigated in detail and proved with biochemical and clinical data.
Subject Keywords
Biochemistry.
URI
http://etd.lib.metu.edu.tr/upload/12611477/index.pdf
https://hdl.handle.net/11511/19295
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
The serum immunoglobulin G glycosylation signature of gastric cancer
Ruhaak, L. Renee; Barkauskas, Donald A.; Torres, Javier; Cooke, Cara L.; Wu, Lauren D.; Stroble, Carol; Özcan Kabasakal, Süreyya; Williams, Cynthia C.; Camorlinga, Margarita; Rocke, David M.; Lebrilla, Carlito B.; Solnick, Jay V. (Elsevier BV, 2015-03-01)
Biomarkers may facilitate detection of gastric cancer at an earlier stage and reduce mortality. Here we sought to determine if the glycosylation profile of serum immunoglobulin G (IgG) could distinguish patients with non-atrophic gastritis (NAG), duodenal ulcer (DU) and gastric cancer (GC). Serum IgG was released and analyzed using nano-LC–TOF mass spectrometry. Statistically significant false discovery rate (FDR)-adjusted p-values were observed for 18 glycans, eight that differed significantly between NAG ...
Human serum arylesterase and glutathione s-transferase activities in patients with ischemic stroke compared to healthy controls
Türkanoğlu, Aysun; Adalı, Orhan; Department of Biochemistry (2007)
Stroke is an important public health problem and the third leading cause of death after coronary heart diesase and all cancers in all over the world. Free radicals and oxidative stress play important role in the pathogenesis of several diseases including atherosclerosis, stroke, cancer, neurodegenerative diseases such as Alzheimer's dementia. The activity of paraoxonase (PON1) aganist phenylacetate is known as arylesterase (ARE). Paraoxonase is an esterase associated with high-density lipoprotein (HDL) and ...
Effects of benzene on liver, kidney and lung cyp1a, cyp2b4, cyp2e1 and cyp3a6 mrna, protein level, and drug metabolizing enzyme activities and toxicity in diabetic rabbits
Arslan, Şevki; Arınç, Emel; Department of Biochemistry (2008)
The effects of diabetes on cytochrome P450 dependent drug metabolizing enzymes have not to be clarified yet. The most widely used animals in these studies have been rats, and information regarding the effects of diabetes on cytochrome P450 dependent procarcinogen/carcinogen metabolism in rabbits is limited. In the present study, we investigated, for the first time, the influence of benzene on liver, kidney and lung microsomal cytochrome P450 dependent drug metabolizing enzyme activities, protein and mRNA le...
Examination of the dielectrophoretic spectra of MCF7 breast cancer cells and leukocytes
Çağlayan, Zeynep; Demircan Yalçın, Yağmur; Külah, Haluk (Wiley, 2020-03-01)
The detection of circulating tumor cells (CTCs) in blood is crucial to assess metastatic progression and to guide therapy. Dielectrophoresis (DEP) is a powerful cell surface marker-free method that allows intrinsic dielectric properties of suspended cells to be exploited for CTC enrichment/isolation from blood. Design of a successful DEP-based CTC enrichment/isolation system requires that the DEP response of the targeted particles should accurately be known. This paper presents a DEP spectrum method to inve...
POSSIBLE INVOLVEMENT OF MANGANESE IN THE CATALYTIC MECHANISM OF BOVINE LIVER ARGINASE
TURKOGLU, S; OZER, I (Elsevier BV, 1992-06-01)
1. Bovine liver arginase followed Michaelis-Menten kinetics in the pH range of 4.5-9.0. The variation of upsilon(i) pH implied that a basic group (pK(alpha) 8.7) functions at the catalytic site.
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
R. Gurbanov, “The effects of selenium on stz-induced diabetic rat kidney plasma membrane,” M.S. - Master of Science, Middle East Technical University, 2010.