Gene Therapy Strategies in Bone Tissue Engineering and Current Clinical Applications

Date

2018-01-01

Author

Atasoy-Zeybek, Aysegul
KÖSE, GAMZE

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

369
views

0
downloads

Gene therapy provides a promising approach for regeneration and repair of injured bone. Application of gene therapy has displayed increased efficiency in various animal models and preclinical trials in comparison with traditional bone grafting methods. The objective of this review is to highlight fundamental principles of gene therapy strategies in bone tissue engineering and solutions of their current limitations for the healing of bone injury. Vector types are debated for the repair of defected site due to demonstration of constraints and applications of the protocols. In recent years, the combination of gene therapy strategies and bone tissue engineering has highly gained attention. We discussed viral and non-viral mediated delivery of therapeutic protein by using scaffolds for bone tissue engineering. Although pre-clinical studies have showed that gene therapy has very promising results to heal injured bone, there are several limitations regarding with the usage of gene delivery methods into clinical applications. Choice of suitable vector, selection of transgene and gene delivery protocols are the most outstanding questions. This article also addresses current state of gene delivery strategies in bone tissue engineering for their potential applications in clinical considerations.

Subject Keywords

Bone tissue engineering, Gene activated matrices, Gene therapy, Viral vectors

URI

https://hdl.handle.net/11511/64943

Journal

CELL BIOLOGY AND TRANSLATIONAL MEDICINE, VOL 4: STEM CELLS AND CELL BASED STRATEGIES IN REGENERATION

DOI

https://doi.org/10.1007/5584_2018_253

Collections

Biomaterials and Tissue Engineering Application and Research Center (BİOMATEN), Article

Suggestions

OpenMETU
Core

Simvastatin loaded porous hydroxyapatite based microcarriers for bone tissue engineering / Güldiken, Merve; Tezcaner, Ayşen; Durucan, Caner; Department of Biotechnology (2014) Bone tissue engineering provides a new medical therapy as an alternative to conventional bone replacement grafts. Carriers designed for bone tissue engineering applications should be biocompatible, bioactive, and porous and should also meet certain minimal requirements to obtain functional engineered tissues. Polymers, ceramic materials and their composites are widely used for developing such carriers. The objective of this study was to develop and characterize a simvastatin (SIM) loaded porous hydroxyapati...
Decellularized Bone Extracellular Matrix-Coated Electrospun PBAT Microfibrous Membranes with Cell Instructive Ability and Improved Bone Tissue Forming Capacity Karakaya, Ece; Erdoğan, Yaşar Kemal; Arslan, Tugba Sezgin; Arslan, Yavuz Emre; Odabaş, Sedat; Ercan, Batur; Emregül, Emel; Derkuş, Burak (2022-09-01) Current approaches to develop bone tissue engineering scaffolds have some limitations and shortcomings. They mainly suffer from combining mechanical stability and bioactivity on the same platform. Synthetic polymers are able to produce mechanically stable sturctures with fibrous morphology when they are electrospun, however, they cannot exhibit bioactivity, which is crucial for tissue engineering and regenerative medicine. One current strategy to bring bioactivity in synthetic materials is to combine extrac...
Decellularized adipose tissue matrix-coated and simvastatin-loaded hydroxyapatite microspheres for bone regeneration Kesim, Merve G.; Durucan, Caner; Atila, Deniz; Keskin, Dilek; Tezcaner, Ayşen (2022-01-01) © 2022 Wiley Periodicals LLC.Simvastatin (SIM)-loaded and human decellularized adipose tissue (DAT)-coated porous hydroxyapatite (HAp) microspheres were developed for the first time to investigate their potential on bone regeneration. Microspheres were loaded with SIM and then coated with DAT for modifying SIM release and improving their biological response. HAp microspheres were prepared by water-in-oil emulsion method using camphene (C10H16) as porogen followed by camphene removal by freeze-drying and sin...
Wet spun PCL scaffolds for tissue engineering Malikmammadov, Elbay; Hasırcı, Nesrin; Endoğan Tanır, Tuğba; Department of Micro and Nanotechnology (2017) Scaffolds produced for tissue engineering applications are promising alternatives to be used in healing and regeneration of injured tissues and organs. In this study, fibrous poly(ε-caprolactone) (PCL) scaffolds were prepared by wet spinning technique and modified by addition of β-tricalcium phosphate (β-TCP) and by immobilizing gelatin onto fibers. Meanwhile, gelatin microspheres carrying Ceftriaxone sodium (CS), a model antibiotic, were added onto the scaffolds and antimicrobial activity of CS was investi...
3D Porous Composite Scaffold of PCL-PEG-PCL/Sr2+ and Mg2+ Ions Co-Doped Borate Hydroxyapatite for Bone Tissue Engineering Yedekçi, Buşra; Evis, Zafer; Tezcaner, Ayşen; Department of Engineering Sciences (2021-9-6) Bioceramic/polymer composite systems have gained importance in treating hard tissue damages using bone tissue engineering (BTE). In this context, it was aimed to develop 3D porous composite PCL-PEG-PCL scaffolds containing different amounts of B, Sr and Mg multi-doped hydroxyapatite (HA) that can provide bone regeneration in the bone defect area and to investigate the effect of both the amount of inorganic phase and the porosity on the mechanical and the biological properties. B-Sr-Mg multi-doped HAs were s...

Citation Formats

A. Atasoy-Zeybek and G. KÖSE, “Gene Therapy Strategies in Bone Tissue Engineering and Current Clinical Applications,” CELL BIOLOGY AND TRANSLATIONAL MEDICINE, VOL 4: STEM CELLS AND CELL BASED STRATEGIES IN REGENERATION, pp. 85–101, 2018, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/64943.